Several genuine Native Americans played important roles in the three-reel melodrama Neola the Sioux. Director E.E. Blackwell plays a young man who attends a wild-west show staged by the Miller brothers' 101 Ranch. While enjoying the spectacle, Blackwell is intrigued by one of the performers, a sad-faced Indian maiden named Neola (Neola Mae). At this point, Chief Eagle Eye (playing himself) fills Blackwell in on the details of Neola's life. It seems that she had been forced into marriage with a white man (Duke Lee) to save him from being killed by her hot-headed sweetheart Red Deer (Pedro Leone). Later deserted by her husband, Neola searches for her missing mate throughout the streets of San Francisco. Meanwhile, Red Deer, now a well-behaved law student, has come to Frisco to attended the 1915 Exposition. Finding Neola in a state of near-starvation, Red Deer arranges for her to work with the Miller brothers as a trick rider. Unfortunately, her no-good white husband returns, threatening to beat Neola to a pulp. Once again, Red Deer comes to the rescue, killing the husband. The courts rule justifiable homicide, whereupon Red Deer is free at last to marry Neola.
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